taq pola (Valiant Co Ltd)
Structured Review

Taq Pola, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taq pola/product/Valiant Co Ltd
Average 94 stars, based on 1504 article reviews
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1) Product Images from "PCR performance of a thermostable heterodimeric archaeal DNA polymerase"
Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2014.00195
Figure Legend Snippet: Properties of experimental thermophilic DNA polymerases .
Techniques Used:
Figure Legend Snippet: Effect of input genomic DNA on PCR efficiency and specificity . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec. The arrow indicates the specific 0.5 kb band.
Techniques Used: Amplification, Molecular Weight
Figure Legend Snippet: Impact of thermal denaturation during cycling . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at the indicated temperature, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec.
Techniques Used: Amplification, Molecular Weight
Figure Legend Snippet: Rate of DNA extension . PCR amplification of the 1.95 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, varying times in seconds as indicated at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec. The arrow indicates the specific 1.95 kb band.
Techniques Used: Amplification, Molecular Weight
Figure Legend Snippet: PCR amplification of DNA fragments of various lengths . PCR reactions were carried out using 100 ng of genomic DNA with 0.1 Uof Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to amplify 0.5, 1.1, 1.95, 2.95, 4.15, and 10 kb (Table ). PCR programs were (2 min at 94°C) × 1; (1 min at 94°C/1 min at 58°C/2, 4, 6, 8, 11, and 16 min with respect to the target length at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec.
Techniques Used: Amplification, Molecular Weight
Figure Legend Snippet: PCR amplification in the presence of primer mismatches . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to introduce 0, 1, 2, 3, and 4 mismatches at the 3'-termini of the forward primer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. The arrow indicates the specific 0.5 kb band.
Techniques Used: Amplification, Introduce
Figure Legend Snippet: Potential inhibitory effects of organic and inorganic substances on PCR .
Techniques Used: Concentration Assay