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Properties of experimental thermophilic DNA polymerases .
Taq Pola, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1504 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "PCR performance of a thermostable heterodimeric archaeal DNA polymerase"

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2014.00195

Properties of experimental thermophilic DNA polymerases .
Figure Legend Snippet: Properties of experimental thermophilic DNA polymerases .

Techniques Used:

Effect of input genomic DNA on PCR efficiency and specificity . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec. The arrow indicates the specific 0.5 kb band.
Figure Legend Snippet: Effect of input genomic DNA on PCR efficiency and specificity . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec. The arrow indicates the specific 0.5 kb band.

Techniques Used: Amplification, Molecular Weight

Impact of thermal denaturation during cycling . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at the indicated temperature, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec.
Figure Legend Snippet: Impact of thermal denaturation during cycling . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at the indicated temperature, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec.

Techniques Used: Amplification, Molecular Weight

Rate of DNA extension . PCR amplification of the 1.95 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, varying times in seconds as indicated at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec. The arrow indicates the specific 1.95 kb band.
Figure Legend Snippet: Rate of DNA extension . PCR amplification of the 1.95 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, varying times in seconds as indicated at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec. The arrow indicates the specific 1.95 kb band.

Techniques Used: Amplification, Molecular Weight

PCR amplification of DNA fragments of various lengths . PCR reactions were carried out using 100 ng of genomic DNA with 0.1 Uof Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to amplify 0.5, 1.1, 1.95, 2.95, 4.15, and 10 kb (Table ). PCR programs were (2 min at 94°C) × 1; (1 min at 94°C/1 min at 58°C/2, 4, 6, 8, 11, and 16 min with respect to the target length at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec.
Figure Legend Snippet: PCR amplification of DNA fragments of various lengths . PCR reactions were carried out using 100 ng of genomic DNA with 0.1 Uof Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to amplify 0.5, 1.1, 1.95, 2.95, 4.15, and 10 kb (Table ). PCR programs were (2 min at 94°C) × 1; (1 min at 94°C/1 min at 58°C/2, 4, 6, 8, 11, and 16 min with respect to the target length at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec.

Techniques Used: Amplification, Molecular Weight

PCR amplification in the presence of primer mismatches . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to introduce 0, 1, 2, 3, and 4 mismatches at the 3'-termini of the forward primer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. The arrow indicates the specific 0.5 kb band.
Figure Legend Snippet: PCR amplification in the presence of primer mismatches . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to introduce 0, 1, 2, 3, and 4 mismatches at the 3'-termini of the forward primer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. The arrow indicates the specific 0.5 kb band.

Techniques Used: Amplification, Introduce

Potential inhibitory effects of organic and inorganic substances on PCR .
Figure Legend Snippet: Potential inhibitory effects of organic and inorganic substances on PCR .

Techniques Used: Concentration Assay



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Valiant Co Ltd taq pola
Properties of experimental thermophilic DNA polymerases .
Taq Pola, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taq pola/product/Valiant Co Ltd
Average 94 stars, based on 1 article reviews
taq pola - by Bioz Stars, 2026-06
94/100 stars
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Properties of experimental thermophilic DNA polymerases .

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: Properties of experimental thermophilic DNA polymerases .

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques:

Effect of input genomic DNA on PCR efficiency and specificity . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec. The arrow indicates the specific 0.5 kb band.

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: Effect of input genomic DNA on PCR efficiency and specificity . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec. The arrow indicates the specific 0.5 kb band.

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques: Amplification, Molecular Weight

Impact of thermal denaturation during cycling . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at the indicated temperature, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec.

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: Impact of thermal denaturation during cycling . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at the indicated temperature, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. Molecular weight markers (M) are SmartLadder SF from Eurogentec.

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques: Amplification, Molecular Weight

Rate of DNA extension . PCR amplification of the 1.95 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, varying times in seconds as indicated at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec. The arrow indicates the specific 1.95 kb band.

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: Rate of DNA extension . PCR amplification of the 1.95 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, varying times in seconds as indicated at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec. The arrow indicates the specific 1.95 kb band.

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques: Amplification, Molecular Weight

PCR amplification of DNA fragments of various lengths . PCR reactions were carried out using 100 ng of genomic DNA with 0.1 Uof Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to amplify 0.5, 1.1, 1.95, 2.95, 4.15, and 10 kb (Table ). PCR programs were (2 min at 94°C) × 1; (1 min at 94°C/1 min at 58°C/2, 4, 6, 8, 11, and 16 min with respect to the target length at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec.

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: PCR amplification of DNA fragments of various lengths . PCR reactions were carried out using 100 ng of genomic DNA with 0.1 Uof Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to amplify 0.5, 1.1, 1.95, 2.95, 4.15, and 10 kb (Table ). PCR programs were (2 min at 94°C) × 1; (1 min at 94°C/1 min at 58°C/2, 4, 6, 8, 11, and 16 min with respect to the target length at 72°C) × 30. Molecular weight markers (M) are SmartLadder LF from Eurogentec.

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques: Amplification, Molecular Weight

PCR amplification in the presence of primer mismatches . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to introduce 0, 1, 2, 3, and 4 mismatches at the 3'-termini of the forward primer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. The arrow indicates the specific 0.5 kb band.

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: PCR amplification in the presence of primer mismatches . PCR amplification of the 0.5 kb target (Table ) was carried out with 0.1 U of Pab-polD (A) , 1 U of Pab-polB (B) , and 1 U of Taq-polA (C) in their respective reaction buffer (Table ). Primer sets were chosen to introduce 0, 1, 2, 3, and 4 mismatches at the 3'-termini of the forward primer (Table ). PCR program was (2 min at 94°C) × 1; (1 min at 94°C, 1 min at 58°C, 2 min at 72°C) × 30; (5 min at 72°C) × 1. The arrow indicates the specific 0.5 kb band.

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques: Amplification, Introduce

Potential inhibitory effects of organic and inorganic substances on PCR .

Journal: Frontiers in Microbiology

Article Title: PCR performance of a thermostable heterodimeric archaeal DNA polymerase

doi: 10.3389/fmicb.2014.00195

Figure Lengend Snippet: Potential inhibitory effects of organic and inorganic substances on PCR .

Article Snippet: Pab-polB ( Isis DNA polymerase) and Taq-polA ( Taq DNA polymerase) were purchased from MP biomedicals.

Techniques: Concentration Assay